An Actinophage for Streptomyces griseus

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Electron microscope study of the multiplication of the actinophage affecting Streptomyces griseus.

Although fundamental problems relating to phage multiplication have been attacked with various genetic, biochemical, and morphologic methods, important questions still remain to be solved especially concerning gene and chromosome replications and DNA synthesis in phages. Recently phages which attack species of Streptomyces have become the subject of study by many workers because of the industri...

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Biochemical characterization of an actinophage for Streptomyces venezuelae.

Actinophage MSP8 has been purified and concentrated by partitioning in a two-phase aqueous polymer system containing methylcellulose and sodium dextran sulfate, by dialysis, by elution chromatography with ECTEOLA-cellulose, and by gel filtration (R. A. Kolstad and S. G. Bradley, J. Bacteriol. 87:1157, 1964). Purified suspensions of the phage contained at least 101i plaque-forming units (PFU) pe...

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Oxidation of Meloxicam by Streptomyces griseus

The aim of the present investigation was to biotransform the anti-inflammatory compound meloxicam by enzymes present in whole cells of five actinomycete cultures to produce novel bioactive derivatives. Among the actinomycetes screened, Streptomyces griseus NCIM 2622 was found to possess the enzyme system(s) that oxidize meloxicam into two metabolites whereas that present in S. griseus NCIM 2623...

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Genetic recombination in Streptomyces griseus.

Low-frequency (10(-6)) genetic recombination was observed in a cephamycin-producing strain of Streptomyces griseus. The recombinants were predominantly heteroclones. Heteroclone analysis was performed involving four heteroclones of one cross. In 100 mutants correlation was found between the type of auxotrophy and the level of antibiotic activity. A cross of this strain with a streptomycin-produ...

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AmfS, an extracellular peptidic morphogen in Streptomyces griseus.

The amf gene cluster was previously identified as a regulator for the onset of aerial-mycelium formation in Streptomyces griseus. The nucleotide sequences of amf and its counterparts in other species revealed a conserved gene organization consisting of five open reading frames. A nonsense mutation in amfS, encoding a 43-amino-acid peptide, caused significant blocking of aerial-mycelium formatio...

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ژورنال

عنوان ژورنال: Journal of Bacteriology

سال: 1947

ISSN: 0021-9193,1098-5530

DOI: 10.1128/jb.54.4.451-466.1947